Cynomolgus CD84 Protein

03.04.2013

Sino Biological Inc. liefert Cynomolgus CD84 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus CD84 Protein lesen Sie bitte: http://www.sinobiological.com/CD84-Protein-Antibody-a-316.html

Produkt-Information von CD84 Protein

Synonym	CD84
Protein Construction	A DNA sequence encoding the cynomolgus CD84 (F6QA53) (Met1-Arg220) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von CD84 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CD84 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 22
Molecular Mass	The recombinant cynomolgus CD84 is a disulfide-linked homodimer. The reduced monomer comprises 440 amino acids and has a calculated molecular mass of 49.2 KDa. The apparent molecular mass of the protein is approximately 57 and 65 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD84 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD84 Protein

The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein ) and EAT-2 ( EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. Human CD84 is expressed as a single chain on dendritic cells, monocytes, macrophages, eosinophils, mast cells, granulocytes, and platelets. As a self-ligand, only the first N-terminal Ig V domain is requisite for the homotypic CD84-CD84 interaction, and it has been demonstrated that CD84 homophilic engagement enhances TCR-induced IFN-γ secretion and induces platelet stimulation.

References

De la Fuente MA. et al., 1997, Blood. 90: 2398-405.
Mmartin M. et al., 2001, J Immunol. 167: 3668-76.
Veillette A. 2006, Nat Rev Immunol. 6: 56-66.
Tangye SG. et al., 2002, Eur J Immunol. 32: 1640-1649.
Nanda N. et al., 2005, Blood. 106: 3028-34.
Yan Q. et al., 2007, Proc Natl Acad Sci. 104: 10583-8.