Cynomolgus NKp80 / KLRF1 Protein

08.06.2013

Sino Biological Inc. liefert Cynomolgus NKp80 / KLRF1 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus NKp80 / KLRF1 Protein lesen Sie bitte:http://www.sinobiological.com/NKP80-KLRF1-Protein-Antibody-a-6220.html

Produkt-Information von NKp80 / KLRF1 Protein

Synonym	KLRF1
Protein Construction	A DNA sequence encoding the cynomolgus KLRF1(Q8MI05) (Val66-Tyr231) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von NKp80 / KLRF1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: KLRF1 protein
Bio-activity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to KLRF1 coated plates (5 μg/mL with 100 μL/well), > 60% cells will adhere for 1 hour incubation at 37℃.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant cynomolgus KLRF1 comprises 426 amino acids and has a calculated molecular mass of 47.8 KDa.The apparent molecular mass of the protein is approximately 59 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NKp80 / KLRF1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NKp80 / KLRF1 Protein

NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor which is expressed on nearly all natural killer cells and stimulates their cytoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y30F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp80, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-70 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.

References

Kuttruff S. et al., 2009, Blood. 113 (2): 358-69.
Dennehy KM. et al., 2011, J Immunol. 186 (2): 657-61.
Roda-Navarro P. et al., 2000, Eur J Immunol. 30 (2): 568-76.