Cynomolgus IGFBP1 Protein (His Tag)

21.09.2012

Produkt-Information von IGFBP1 Protein

Synonym	IGFBP1
Protein Construction	A DNA sequence encoding the cynomolgus IGFBP1 (F6RF22) (Met23-Asn281) was expressed, fused with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von IGFBP1 Protein

Purity	> 98 % as determined by SDS-PAGE	SDS-PAGE: IGFBP1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 48
Molecular Mass	The recombinant Cynomolgus IGFBP1 is a disulfide-linked homodimer. The reduced monomer comprises 245 amino acids and has a calculated molecular mass of 26.7 KDa. The apparent molecular mass of Cynomolgus IGFBP1 heterodimer is approximately 14 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IGFBP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IGFBP1 Protein

IGFBP1, also known as IGFBP-1 and insulin-like growth factor-binding protein 1, belongs to the insulin-like growth factor binding protein family. IGF binding proteins (IGFBPs) are proteins of 24 to 45 kDa. All six IGFBPs share 50% homology with each other and have binding affinities for IGF-I and IGF-II at the same order of magnitude as the ligands have for the IGF-IR.IGFBP1 is a protein with an IGFBP domain and a type-I thyroglobulin domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP1 promotes cell migration.

References

Wood AW. et al., 2005, Int Rev Cytol. 243: 215-85.
Firth SM. et al., 2003, Endocr Rev. 23 (6): 824-54.
Ferry RJ. et al., 1999, Horm Res. 51 (2): 53-67.

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