Cynomolgus CD86 / B7-2 Protein

22.05.2013

Sino Biological Inc. liefert Cynomolgus CD86 / B7-2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus CD86 / B7-2 Protein lesen Sie bitte: http://www.sinobiological.com/CD86-B7-2-Protein-Antibody-a-317.html

Produkt-Information von CD86 / B7-2 Protein

Synonym	CD86
Protein Construction	A DNA sequence encoding the cynomolgus CD86 (Q9BDM4) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von CD86 / B7-2 Protein

Purity	(87.0+5.3)% as determined by SDS-PAGE	SDS-PAGE: CD86 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 20
Molecular Mass	The recombinant cynomolgus CD86 is a disulfide-linked homodimer. The reduced monomer comprises 461 amino acids and has a calculated molecular mass of 52.2 KDa.The apparent molecular mass of the protein is approximately 76-86 and 34 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD86 / B7-2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD86 / B7-2 Protein

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte -associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. CD28 has two ligands expressed largely on professional APC, named B7-1 (CD80) and B7-2 (CD86). They are both type I transmembrane proteins with a membrane distal IgV and a membrane proximal Ig constant (IgC) domain and share 25% sequence identity. Although interacting with the same receptors, B7-1 and B7-2 show several distinct features. Binding of B7-2 with CD28 induces the activation of T cell, whereas CTLA-4 acts as a negative regulator and diminishes the immune responses. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

References

Freeman GJ. et al., 1993, J Exp Med. 178 (6): 2185-92.
Mark DA. et al., 1998, Int Immunol. 10 (11): 1647-55.
Girndt M. et al., 2001, Kidney Int. 59 (4): 1382-9.
Tikkanen JM. et al., 2002, Am J Respir Crit Care Med. 165 (5): 724-9.
O'Neill SK. et al., 2007, J Immunol. 179 (8): 5109-16.
Salek-Ardakani S. et al., 2009, J Immunol. 82 (5): 1909-18.