Carbonic Anhydrase IX / CA9 Antikörper

Sino Biological Inc. liefert Carbonic Anhydrase IX / CA9 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Carbonic Anhydrase IX / CA9 Antikörper lesen Sie bitte:http://www.sinobiological.com/Carbonic-Anhydrase-IX-Protein-Antibody-a-543.html

Produkt-Information von Carbonic Anhydrase IX / CA9 Antikörper

Usage Guide von Carbonic Anhydrase IX / CA9 Antikörper

Specificity	Human Carbonic Anhydrase IX / CA9
Flow Cytometry	Flow cytometric analysis of CA9 expression on HeLa cells. HeLa cells were stained with anti-CA9 Monoclonal Ab (10107-R036, 1 μg/test), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA9.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Carbonic Anhydrase IX / CA9 Antikörper

Carbonic anhydrases IX (CAIX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CAIX is a transmembrane protein structurally consisting of a signal peptide, a proteoglycan-related region, a CA domain with a highly conserved active site, a transmembrane anchor and an intracytoplasmic tail, and is the only tumor-associated CA isoenzyme known so far. Compared with normal tissues, CAIX is overexpressed in a wide spectrum of tumor types and associated with increased metastasis and poor prognosis in aggressive carcinomas. CAIX expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. CA9 is thus regarded as a new therapeutic target for CA9-derived carcinomas.

References

1. Pastorek, J. et al., 1994, Oncogene. 9: 2877-2888.
2. Opavsky, R. et al., 1996, Genomics. 33: 480-487.
3. Swietach, P. et al., 2008, J. Biol. Chem. 283: 20473-20483.
4. Robertson, N. et al., 2004, Cancer. Res. 64: 6160-6165.
5. Bui, M.H. et al., 2003, Clin. Cancer. Res. 9: 802-811.
6. Choi, S.W. et al., 2008, Hum. Pathol. 39: 1317-1322.