CRIP2 Protein (GST Tag)

18.03.2013

Sino Biological Inc. liefert CRIP2 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CRIP2 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/CRIP2-Protein-Antibody-a-5723.html

Produkt-Information von CRIP2 Protein

Synonym	CRIP2, CRP2
Protein Construction	A DNA sequence encoding the human CRIP2 (P52943) (Met1-Pro208) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CRIP2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CRIP2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CRIP2/GST chimera consists of 445 amino acids and has a calculated molecular mass of 50.3 kDa. The recombinant protein migrates as an approximately 49 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CRIP2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CRIP2 Protein

CRIP2 is a putative transcription factor. It has a widespread tissue expression and is highly expressed in heart. CRIP2 contains two LIM zinc-binding domains. CRIP2 may participate in the differentiation of smooth muscle tissue. It also plays an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis. It interacts with the NF-κB/p65 to inhibit its DNA-binding ability to the promoter regions of the major proangiogenesis cytokines critical for tumor progression, including IL6, IL8, and VEGF. In conclusion, we provide compelling evidence that CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis.

References

Chang DF. et al., 2003, Dev Cell. 4 (1):107-18.
Huber A. et al., 2000, J Biol Chem. 275 (8): 5504-11.
Karim MA. et al., 1996, Genomics. 31 (2): 167-76.