ASF1B Protein (His Tag)

15.03.2013

Sino Biological Inc. liefert ASF1B Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ASF1B Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ASF1B-Protein-g-10613.html

Produkt-Information von ASF1B Protein

Synonym	ASF1B
Protein Construction	A DNA sequence encoding the human ASF1B (Q9NVP2)( Met 1- Ile202) was expressed with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ASF1B Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ASF1B protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human ASF1B consists of 212 amino acids and predicts a molecular mass of 23.8 KDa. The apparent molecular mass of the protein is approximately 20 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ASF1B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ASF1B Protein

ASF1B is a member of the ASF1 family. It is a histone chaperone protein and highly expressed in testis while at lower levels in colon, small intestine and thymus. ASF1B may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. It facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. ASF1B interacts with histone H3 and histone H4. It is required for spermatogenesis. ASF1B also is the substrate of the tousled-like kinase family of cell cycle-regulated kinases.

References

Sen SP. et al., 2006, BMC Mol Biol. 7: 37.
Groth A. et al., 2005, Mol Cell. 17 (2): 301-11.
Wei H. et al., 2005, Nat Biotechnol. 22 (6): 707-16.