EIF3K Protein (GST Tag)

18.03.2013

Sino Biological Inc. liefert EIF3K Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über EIF3K Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/EIF3K-Protein-Antibody-a-5725.html

Produkt-Information von EIF3K Protein

Synonym	EIF3K, EIF3S12
Protein Construction	A DNA sequence encoding the human EIF3K (Q9UBQ5)(Ala2-Gln218) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von EIF3K Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: EIF3K protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human EIF3K consists of 454 amino acids and predicts a molecular mass of 52.8 KDa. The apparent molecular mass of the protein is approximately 47 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 3mM DTT, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von EIF3K Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von EIF3K Protein

EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 40S ribosomal subunit.

References

Rual JF. et al., 2005, Nature. 437 (7062): 1173-8.
Shen X. et al., 2004, FEBS Lett. 573 (1-3): 139-46.
Wei Z. et al., 2004, J Biol Chem. 279 (33): 34983-90.