CPNE1 / Copine I / CPN1 Protein (N-SUMO Tag)

10.01.2013

Sino Biological Inc. liefert CPNE1 / Copine I / CPN1 Protein (N-SUMO Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CPNE1 / Copine I / CPN1 Protein (N-SUMO Tag) lesen Sie bitte: http://www.sinobiological.com/CPNE1-Copine-I-CPN1-Protein-g-10322.html

Produkt-Information von CPNE1 / Copine I / CPN1 Protein

Synonym	CPNE1, CPN1
Protein Construction	A DNA sequence encoding the human CPNE1(Q99829) (Met1-Ala537) was fused with the SUMO tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CPNE1 / Copine I / CPN1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CPNE1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human CPNE1 consists of 656 amino acids and predicts a molecular mass of 72.7 KDa. The apparent molecular mass of the protein is approximately 73 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CPNE1 / Copine I / CPN1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CPNE1 / Copine I / CPN1 Protein

Copine I, also known as CPN1, is a member of the copine family. Copine I is a calcium-dependent membrane-binding protein which has a wide tissue distribution. Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. Copine I contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus, while it does not contain a predicted signal sequence or transmembrane domains. Copine I may function in membrane trafficking.

References

Cowland JB. et al., 2003, J Leukoc Biol. 74 (3): 379-88.
Tomsig JL. et al., 2003, J Biol Chem. 278 (12): 10048-54.
Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.