CNPY2 Protein (His Tag)

09.01.2013

Sino Biological Inc. liefert CNPY2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CNPY2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CNPY2-Protein-g-10317.html

Produkt-Information von CNPY2 Protein

Synonym	CNPY2, MSAP, TMEM4, ZSIG9
Protein Construction	A DNA sequence encoding the human CNPY2 (Q9Y2B0-1) (Met1-Ser178) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CNPY2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CNPY2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Arg 21
Molecular Mass	The recombinant human CNPY2 consists of 173 amino acids and predicts a molecular mass of 20 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CNPY2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CNPY2 Protein

CNPY2 is a novel MIR-interacting protein. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein, and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 is expressed in different tissues, including brain. Overexpression of CNPY2 enhanced the motility of glioma cells measured in matrigel invasion chambers and using a scratch assay. CNPY2 enhances neurite outgrowth and increases myosin regulatory light chain. It also enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain. Inhibition of the corresponding MRLC kinase by ML-7 did not affect migration of CNPY2-overexpressing cells.

References

Trynka G. et al., 2009, Gut. 58 (8): 1078-83.
Danielsen JM. et al., 2011, Mol Cell Proteomics. 10 (3): M110.003590.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.