Glutaminyl cyclase / QPCT Protein (His Tag)

09.01.2013

Sino Biological Inc. liefert Glutaminyl cyclase / QPCT Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Glutaminyl cyclase / QPCT Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Glutaminyl-cyclase-QPCT-Protein-g-10316.html

Produkt-Information von Glutaminyl cyclase / QPCT Protein

Synonym	QPCT
Protein Construction	A DNA sequence encoding the human QPCT (Q16769-1)(Ala33-Leu361) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Glutaminyl cyclase / QPCT Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: QPCT protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human QPCT consists of 345 amino acids and has a calculated molecular mass of 39.7 kDa. The recombinant protein migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4,10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Glutaminyl cyclase / QPCT Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Glutaminyl cyclase / QPCT Protein

Glutaminyl cyclase, also known as QPCT, is an enzyme catalyzing the formation of pyroglutamic peptides or proteins from an N-terminal glutamine residue. The N-terminal pyroglutamate(pGlu) formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase’s structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.

References

Busby WH. et al., 1987, J Biol Chem. 262 (18): 8532-6.
Bateman RC. et al., 2001, Biochemistry. 40 (37): 11246-50.
Schilling S. et al., 2002, Biochemistry. 41 (35): 10849-57.