CKB / Creatine kinase B type Protein (His Tag)

24.04.2013

Sino Biological Inc. liefert CKB / Creatine kinase B type Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CKB / Creatine kinase B type Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CKB-Creatine-kinase-B-type-Protein-Antibody-a-5846.html

Produkt-Information von CKB / Creatine kinase B type Protein

Synonym	CKB, CKBB
Protein Construction	A DNA sequence encoding the human CKB (P12277)(Pro2-Lys281) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CKB / Creatine kinase B type Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: CKB protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CKB consists of 398 amino acids and has a calculated molecular mass of 44.8 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CKB / Creatine kinase B type Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CKB / Creatine kinase B type Protein

CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

References

Wienker TF. et al., 1985, Cytogenet Cell Genet. 40: 776.
Kotz R. et al., 1977 Wien Klin Wochenschr. 89 (14): 474-9.
Mariman EC. et al., 1989, Nucleic Acids Res. 17 (15): 6385.
Bong S. et al., 2008, FEBS Letters. 582 (28): 3959-65.

CKB (Creatine kinase B type) related areas, pathways, and other information