SRFBP1 Protein (GST Tag)

24.04.2013

Sino Biological Inc. liefert SRFBP1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SRFBP1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/SRFBP1-p49-STRAP-Protein-Antibody-a-5847.html

Produkt-Information von SRFBP1 Protein

Synonym	SRFBP1
Protein Construction	A DNA sequence encoding the human SRFBP1(Q8NEF9) (Met1-Asp429) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von SRFBP1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: SRFBP1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human SRFBP1 consists of 666 amino acids and predicts a molecular mass of 76.5 KDa. The apparent molecular mass of the protein is approximately 69 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SRFBP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SRFBP1 Protein

SRFBP1 contains 7 WD repeats and belongs to the WD repeat STRAP family. SRFBP1 may play a role in the cellular distribution of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. SRFBP1 negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein. SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. It also may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells.

References

Datta PK. et al., 1999, J Biol Chem. 273 (52): 34671-4.
Datta PK. et al., 1998, J Biol Chem. 273 (52): 34671-4.
Datta PK. et al., 2000, Mol Cell Biol. 20 (9): 3157-67.

SRFBP1 (p49, STRAP) related areas, pathways, and other information