EF1B / EEF1B2 Protein (His Tag)

28.05.2013

Sino Biological Inc. liefert EF1B / EEF1B2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über EF1B / EEF1B2 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/EF1B-EEF1B2-Protein-Antibody-a-6206.html

Produkt-Information von EF1B / EEF1B2 Protein

Synonym	EEF1B2, EEF1B, EF1B
Protein Construction	A DNA sequence encoding the human EEF1B2 (P24534)(Met1-Ile225) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von EF1B / EEF1B2 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: EF1B protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human EEF1B2 consists of 240 amino acids and predicts a molecular mass of 26.6 KDa. It migrates as an approximately 30 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, 1mM DTT, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von EF1B / EEF1B2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von EF1B / EEF1B2 Protein

EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.

References

Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62.
Rual. et al., 2005, Nature. 437 (7062): 1173-8.
Stelzl. et al., 2005, Cell. 122 (6): 957-68.
Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.