CD44 Antikörper

11.05.2012

Produkt-Information von CD44 Antikörper

Immunogen	Recombinant Human CD44 protein (Catalog#12211-H08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	1C11H1
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von CD44 Antikörper

Specificity

Human CD44

No cross-reactivity in ELISA with

Human DMP1

Human cell lysate (293 cell line)

Immunochemistry

IHC-P: 10-30 μg/mL

CD44-bladder-carcinoma immunohistochemistry CD44-skin immunohistochemistry

Immunochemical staining of human CD44 in human bladder carcinoma with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.

Immunochemical staining of human CD44 in human skin with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.

CD44-esophagus immunohistochemistry

Immunochemical staining of human CD44 in human esophagus with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.

Direct ELISA

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD44.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von CD44 Antikörper

CD44, short for Cluster of Differentiation 44, is an adhesion molecule of the hyaluronate receptor family. It is expressed in a large number of mammalian cell types. The adhesive function of CD44, like other molecules involved in inducible adhesion, is tightly regulated. Post-translational modifications, isoform expression, aggregation state, and protein associations all can affect the ligand binding properties of CD44, and these can vary depending on the cell type and the activation state of the cell. The principal ligand of CD44 is hyaluronan, and CD44 is involved in the metabolism of this compound. Furthermore, an increasing quantity of evidence suggests that CD44 has various functions related to inflammatory disease. CD44 deficiency induces severe liver injury. CD44-hyaluronate mediates in lymphocyte T and monocyte adhesion to the endothelium, stimulates proinflammatory cytokine release from macrophages and participates in dedifferentiation phenotype of smooth muscle cells from contractile state to synthetic one. CD44 acts in atherosclerotic plaque destabilization and neointimal proliferation after percutaneous vascular interventions. CD44 participates in many stages of atherosclerosis. CD44 also is a cell membrane protein often overexpressed on tumor cells and, being both a cell-cell and cell-extracellular matrix adhesion protein, is well positioned to contribute to this process.

References

Krettek A. et al.,2009, Cardiovasc Hematol Disord Drug Targets. 9 (4): 293-302.
Johnson P. et al., 2000, Biochem Pharmacol. 59 (5): 455-65.
Kimura K. et al., 2010, Curr Opin Drug Discov Devel. 13 (1): 96-103.
Protasiewicz J. et al., 2005, Pol Merkur Lekarski. 19 (112): 559-62.
Jothy S. 2003, Clin Exp Metastasis. 20 (3): 195-201.

Sino Biological Inc. liefert CD44 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD44 Antikörper lesen Sie bitte: http://www.sinobiological.com/CD44-Antibody-g-8783.html

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
CD44	Human	CD44 Protein, Recombinant	12211-H08H

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human CD44	WB, ELISA	CD44 Antibody (Antigen Affinity Purified)	12211-RP02
Human CD44	ELISA, IHC-P	CD44 Antibody	12211-MM01
Human CD44	FCM	CD44 Antibody ( FITC )	12211-MM02-F
Human CD44	WB, ELISA	CD44 Antibody	12211-MM10

CD44 Antikörper

Produkt-Information von CD44 Antikörper

Usage Guide von CD44 Antikörper

Verwandte Produkte & Themen von CD44 Antikörper

Hintergrund von CD44 Antikörper

References