CD4 Antikörper

08.05.2012

Produkt-Information von CD4 Antikörper

Immunogen	Recombinant Human CD4 protein (Catalog#10400-H08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	113
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met 1-Trp 390).

Usage Guide von CD4 Antikörper

Specificity

Human CD4

No cross-reactivity in ELISA with

Mouse CD4

Human CD32a

Human CD32b

Human CD16a

Human CD16b

Human CD64

Immunochemistry

IHC-P: 1-10 μg/mL

CD4-tonsil immunohistochemistry CD4-lymphoid-node immunohistochemistry

Immunochemical staining of human CD4 in human tonsil with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells.

Immunochemical staining of human CD4 in human lymphoid node with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD4 in WB.

Direct ELISA

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD4.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von CD4 Antikörper

T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoproteinexpressed on the surface of T helper cells, regulatory T cells,monocytes, macrophages, anddendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 before being named CD4 in 1984. CD4 is aco-receptorthat assists theT cell receptor (TCR) to activate its T cell following an interaction with anantigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly withMHC class II molecules on the surface of the antigen presenting cell via itsextracellulardomain. CD4 is a primary receptor used byHIV-1to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

References

Ryu SE, et al., 1994, Structure2 (1): 59-74.
Isobe M, et al., 1986, Proc. Natl. Acad. Sci.83 (12): 4399-402.
Ansari-Lari MA, et al., 1996, Genome Res.6 (4): 314-26.
Hawash, et al., 2002, Biochim. Biophys. Acta. 1589 (2): 140-50.

Sino Biological Inc. liefert CD4 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD4 Antikörper lesen Sie bitte: http://www.sinobiological.com/CD4-Antibody-g-8766.html

CD4 Antikörper

Produkt-Information von CD4 Antikörper

Usage Guide von CD4 Antikörper

Verwandte Produkte & Themen von CD4 Antikörper

Hintergrund von CD4 Antikörper

References