IL-33 Antikörper

08.05.2012

Produkt-Information von IL-33 Antikörper

Immunogen	Recombinant Human IL-33 protein (Catalog#10368-HNAE)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	1D1C5A12
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_002296.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von IL-33 Antikörper

Specificity

Human IL-33

Immunochemistry

IHC-P: 10-20 μg/mL

IL33-lymphoid-node immunohistochemistry

Immunochemical staining of human IL33 in human lymphoid-node with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nuclear of high endothelial venule cells.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von IL-33 Antikörper

Related Areas:

Immunology>>Adaptive Immunity>>T Cell>>Helper T Cell>>IL-33

Immunology>>Cytokine & Receptor>>Interleukin & Receptor >>IL-1 superfamily and IL-1R/TLR family>>IL-33

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
IL-33	Human	IL-33/NF-HEV Protein, Recombinant	10368-HNAE
IL-33	Canine	IL33 / Interleukin-33 / NF-HEV Protein, Recombinant, with Native	70005-DNAE

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human IL-33	WB	Mouse Monoclonal Antibody	10368-MM01
Human IL-33	ELISA	Mouse Monoclonal Antibody	10368-MM02
Human IL-33	IHC-P	IL-33 Antibody	10368-MM13
Human IL-33	WB, ELISA	Rabbit Polyclonal Antibody	10368-RP01
Human IL-33	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10368-RP02

Hintergrund von IL-33 Antikörper

Interleukin-33 (IL-33), initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules, also known as DVS 27, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T (H)2-associated cytokines from in vitro polarized T (H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

References

Cayrol C,Girard JP, 2009, Proc Natl Acad Sci U S A. 106 (22): 9021-6.
Carriere V, et al., 2007, Proc Natl Acad Sci U S A. 104 (1): 282-7.
Baekkevold, E.S. et al., 2003, Am. J. Pathol. 163:69.
Schmitz, J. et al., 2005, Immunity. 23:479.
Black, R.A. et al., 1989, J. Biol. Chem. 264: 5323.
Xu, D. et al., 1998, J. Exp. Med. 187: 787.
Dinarello, C.A., 2005, Immunity. 23: 461.

Sino Biological Inc. liefert IL-33 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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