CD3d / CD3 delta Antikörper

08.05.2012

Produkt-Information von CD3d / CD3 delta Antikörper

Immunogen	Recombinant Human CD3d / CD3 delta protein (Catalog#10981-H08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	6B1C12F3
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3d / CD3 delta (rh CD3d / CD3 delta; Catalog#10981-H08H; NP_000723.1; Met 1-Ala 105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von CD3d / CD3 delta Antikörper

Specificity

Human CD3d / CD3 delta

Immunochemistry

IHC-P: 5-20 μg/mL

CD3D-tonsil immunohistochemistry

Immunochemical staining of human CD3D in human tonsil with mouse Mab (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

CD3D-spleen immunohistochemistry

Immunochemical staining of human CD3D in human spleen with mouse Mab (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von CD3d / CD3 delta Antikörper

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown tointeractwithCD8A. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

References

Doucey, MA. et al., 2003, J. Biol. Chem. 278 (5): 3257-64.
Call, ME. et al., 2004, EMBO J. 23 (12): 2348-57.
Arnett, KL. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 16268-73.
Kjer-Nielsen, L. et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101: 7675-80.
Fischer, A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
Holst, J. et al., 2008, Nature immunology. 9 (6): 658-66.

Sino Biological Inc. liefert CD3d / CD3 delta Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD3d / CD3 delta Antikörper lesen Sie bitte: http://www.sinobiological.com/CD3d-CD3-delta-Antibody-g-8765.html

CD3d / CD3 delta Antikörper

Produkt-Information von CD3d / CD3 delta Antikörper

Usage Guide von CD3d / CD3 delta Antikörper

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Hintergrund von CD3d / CD3 delta Antikörper

References