CD200 Antikörper

Produkt-Information von CD200 Antikörper

Usage Guide von CD200 Antikörper

Specificity	Mouse CD200
Flow Cytometry	BALB/c splenocytes were stained with Mouse CD200 Monoclonal Ab (50074-R002), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes. Flow cytometric analysis of CD200 expression on spleen lymphocytes. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CD200 in WB.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von CD200 Antikörper

Immunology>>Adaptive Immunity>>T Cell>>T Cell Migration/Adhesion>>CD200

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD200

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>T Cell Migration/Adhesion>>CD200

Hintergrund von CD200 Antikörper

CD200, also known as OX-2, is a transmembrane immunoregulatory protein belonging to the immunoglobulin superfamily. The mouse CD200 cDNA encodes a 278 aa precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain, and shares 76% and 94% aa sequence identity with human and rat CD200. CD200, a type 2 transmembrane molecule, plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion by delivering an immunoregulatory signal through interaction with its receptor. CD200wasfoundto beco-expressed with stem cell markers found on prostate, breast, brain, and colon cancers. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact. In T cells, CD200 functions as a costimulatory molecule independent of the CD28 pathway. CD200 and its interaction with the CD200 receptor is a potential therapeutic target for metastatic melanoma (MM).

References

1. Chen, Z. et al., 1997, Biochim Biophys Acta. 1362 (1):6-10.
2. Gorczynski, RM. et al., 2001, Clin Exp Immunol. 126 (2): 220-229.
3. Shiratori, I. et al., 2005, J Immunol. 175 (7): 4441-4449.
4. Kawasaki, BT. et al., 2007, Biochem Biophys Res Commun. 364 (4): 778-782.
5. Kretz-Rommel, A. et al., 2008, J Immunol. 180 (2): 699-705.
6. Chen, Z. et al., 2009, Mol Immunol. 46 (10): 1951-1963.

Sino Biological Inc. liefert CD200 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD200 Antikörper lesen Sie bitte: http://www.sinobiological.com/CD200-Antibody-g-8797.html