CD20 / MS4A1 (aa 141-184) Protein

27.03.2012

Produkt-Information von CD20/MS4A1 Protein

Synonym	B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7
Protein Construction	A DNA sequence encoding the amino acid sequence (Ile 141-Tyr 184) of human CD20 (P11836), corresponding to the epitope 2& 3,was fused with the Fc region of mouse IgG1at the N-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CD20/MS4A1 Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: CD20/MS4A1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp
Molecular Mass	The recombinant human CD20 (aa 141-184) /mFc is a disulfide-linked homodimer. The reduced monomer comprises 280 amino acids and predicts a molecular mass of 31.8 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

CD20/MS4A1 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD20/MS4A1 Protein

B-lymphocyte antigen CD20, also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is a multi-pass membrane protein which belongs to theMS4A family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.

References

Tedder TF. et al.,1988, Proc Natl Acad Sci. 85 (1): 208-12.
Cragg MS. et al., 2005, Curr Dir Autoimmun. 8: 140-74.
Fang D. et al., 2005, Cancer Res. 65 (20): 9328-37.
Teeling JL. et al., 2006, J Immunol. 177: 362-371.

Sino Biological Inc. liefert CD20 / MS4A1 (aa 141-184) Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD20 / MS4A1 (aa 141-184) Protein lesen Sie bitte: http://www.sinobiological.com/CD20-MS4A1-Protein-g-8626.html

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat No
Ferret CD20/MS4A1	WB, ELISA	CD20 / MS4A1 Antibody (Antigen Affinity Purified)	60004-RP02