ASGR1 Protein (His Tag)

27.03.2012

Produkt-Information von ASGR1 Protein

Synonym	ASGPR, ASGPR1, CLEC4H1, HL-1
Protein Construction	A DNA sequence encoding the human ASGR1 isoform 1 (P07306) (Gln 62- Leu 291) was expressed, with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von ASGR1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ASGR1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ASGR1 consists of 246 amino acids and has a calculated molecular mass of 28.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhASGR1 is approximately 37 kDa due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

ASGR1 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ASGR1 Protein

Asialoglycoprotein receptor (ASGPR) is a heterooligomeric receptor that is abundantly expressed on the sinusoidal surface of the hepatic plasma membrane. It is an endocytic receptor that rapidly binds and internalizes galactose-terminated glycoproteins (asialoglycoproteins or ASGP) from the circulation. The mouse ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The aa sequence of mouse ASGPR1 ECD is 89% and 79% identical to the ASGPR1 ECD of rat and human, respectively.

References

Sanford, J. P. et al., 1988, DNA 7:721-728.
Takezawa, R. et al., 1993, Biochim. Biophys. Acta 1172 :220-222.
Monroe, R. S. et al., 1994, Gene 148: 237-244.
Stockert, R. J., 1995, Physiol. Rev. 75(3): 591–609.
Braun, J. R. et al., 1996,?J Biol Chem.?271?(35): 21160-21166.
Meier, M. et al., 2000, J. Mol. Biol. 300: 857-865.
Weigel, P. H. et al., 2002, Biochim. Biophys. Acta 1572:341-363.

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