BMPR1B / ALK6 (149-502) Protein (GST Tag)

27.03.2012

Produkt-Information von BMPR1B / ALK6 Protein

Synonym	BMPR1B,ALK-6, ALK6, CDw293
Protein Construction	A DNA sequence encoding the human ALK6 (NP_001194.1) cytoplasmic domain (Arg 149-Leu 502) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von BMPR1B / ALK6 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: BMPR1B / ALK6 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human ALK6 (149-502)/GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 KDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 50mM Tris,100mM NaCl, pH 8.5, 20%gly, 0.3mM DTT Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

BMPR1B / ALK6 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von BMPR1B / ALK6 Protein

BMPR1B(bone morphogenetic protein receptor, type IB,,His&GST-tagged), also known as ALK6, is a a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. It contains 1 GS domain and 1 protein kinase domain. In the chick embryo, it has been shown that BMPR1B is found in precartilaginous condensations. BMPR1B is the major transducer of signals in these condensations. On ligand binding, it forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. BMPR1B also is a receptor for BMP7/OP-1 and GDF5. The BMPR1B receptor plays a role in the formation of middle and proximal phalanges. Defects in BMPR1B are the cause of acromesomelic chondrodysplasia with genital anomalies and brachydactyly type A2.

References

Ide H. et al., 1998, Cell Genet. 81 (3-4): 285-6.
Mishina Y. et al., 2004, J Biol Chem. 279 (26): 27560-6.
Yoon BS. et al., 2005, Proc Natl Acad Sci. 102 (14): 5062-7.

Sino Biological Inc. liefert BMPR1B / ALK6 (149-502) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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