CD16-2 / FCGR4 Antikörper ( FITC )

Produkt-Information von CD16-2 / FCGR4 Antikörper

Usage Guide von CD16-2 / FCGR4 Antikörper

Specificity	Mouse CD16-2 / FCGR4
	Has cross-reactivity in ELISA with Human CD64 No cross-reactivity in ELISA with Human CD16a Human CD16b Human CD32a Human CD32b Mouse FCGR3
Flow Cytometry	Analysis of FCGR4 expression on Raw264.7 cells. Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse FCGR4 in WB. Using a DAB detection system, the detection limit for Mouse FCGR4 is approximately 0.125 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

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CD16-2 / FCGR4 Antikörper

Receptors for the Fc region of IgG (Fc γ Rs) constitute a subfamily of the Ig superfamily, and function in the activation or inhibition of immune responses. Based on close relationships in their extracellular domains, the Fc γ R have been divided into three classes designated as Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Human CD16 is encoded by two genes: Fc γ RIIIA and Fc γ RIIIB, and the newly described mouse FCGR4, also referred to as CD16-2, has been proposed to be the mouse homolog of human Fc γ RIIIA because of high sequence homology (60 % aa identity). FCGR4 is expressed in peripheral blood leukocytes, spleen, thymus, colon and intestine, and serves as an activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory protein. Although mouse FCGR4 functioned as a low-affinity IgE receptor for all IgE allotypes, ligation of FCGR4 by antigen-IgE (b) immune complexes promotes macrophage-mediated phagocytosis, presentation of antigen to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions.

References

1. Mancardi D.A. et al., 2008, J Clin Invest. 118: 3738-3750.
2. Minard-Colin V. et al., 2008, Blood. 112: 1205-1213.
3. Baudino L. et al., 2008, J Immunol. 180: 1948-1953.
4. Hirano M. et al., 2007, Nat Immunol. 8: 762-771.
5. Jakus Z. et al., 2008, J Immunol. 180: 618-629.

Sino Biological Inc. liefert CD16-2 / FCGR4 Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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