CCDC47 Protein (His Tag)

07.12.2012

Sino Biological Inc. liefert CCDC47 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CCDC47 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CCDC47-Protein-g-9973.html

Produkt-Information von CCDC47 Protein

Synonym	CCDC47
Protein Construction	A DNA sequence encoding the human CCDC47 (Q96A33-1) (Met1-Ser135) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CCDC47 Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: CCDC47 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 21
Molecular Mass	The recombinant human CCDC47 consists of 126 amino acids and predicts a molecular mass of 14.7 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CCDC47 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CCDC47 Protein

CCDC47 gene contains 16 distinct gt-ag introns. Transcription produces 9 different mRNAs, 6 alternatively spliced variants and 3 unspliced forms. There are 3 probable alternative promotors, 3 non overlapping alternative last exons and 8 validated alternative polyadenylation sites. CCDC47 gene is expressed at high level. The gene The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon, overlapping exons with different boundaries. Functionally, CCDC47 gene has been proposed to participate in processes such as calcium ion homeostasis, embryo development, ER overload response and post-embryonic development. CCDC47 are expected to have molecular function (calcium ion binding) and to localize in various compartments (membrane, endoplasmic reticulum, integral to membrane, microsome.

References

Li S. et al., 2011, Immunity. 35 (3): 426-40.
Danielsen JM. et al., 2011, Mol Cell Proteomics. 10 (3): M110.003590.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.