MKI67 Protein (GST Tag)

07.12.2012

Sino Biological Inc. liefert MKI67 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über MKI67 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/MKI67-Protein-9972.html

Produkt-Information von MKI67 Protein

Synonym	MKI67
Protein Construction	A DNA sequence encoding the mature form of human MKI67 (P46013-2) (Met1-Pro120) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von MKI67 Protein

Purity	> 80 % as determined by SDS-PAGE	SDS-PAGE: MKI67 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human MKI67 /GST chimera consists of 354 amino acids and has a predicted molecular mass of 41 kDa. It migrates as an approximately 37-41 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MKI67 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MKI67 Protein

MKI67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. It is also involved in ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis. MKI67 is known as a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. It is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer.

References

Rahmanzadeh R. et al., 2007, Cell Prolif. 40 (3): 422-30.
Bullwinkel J. et al., 2006, J Cell Physiol. 206 (3): 624-35.
Schonk DM. et al., 1989, Hum Genet. 83 (3): 297-9.