AMY2A / Alpha-amylase Protein(His Tag)

07.12.2012

Sino Biological Inc. liefert AMY2A / Alpha-amylase Protein(His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über AMY2A / Alpha-amylase Protein(His Tag) lesen Sie bitte: http://www.sinobiological.com/AMY2A-Alpha-amylase-Protein-g-9974.html

Produkt-Information von AMY2A / Alpha-amylase Protein

Synonym	AMY2A
Protein Construction	A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von AMY2A / Alpha-amylase Protein

Purity	> 85% as determined by SDS-PAGE	SDS-PAGE: AMY2A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 16
Molecular Mass	The recombinant human AMY2A consists of 507 amino acids and predicts a molecular mass of 57.3 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von AMY2A / Alpha-amylase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von AMY2A / Alpha-amylase Protein

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase is the major form of amylase found in humans and other mammals. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.

References

Abe A. et al., 2005, FEBS J. 272 (23): 6145-53.
Aghajari N. et al., 1998, Protein Sci. 7 (3): 564-72.
Ramasubbu N. et al., 1996, Acta Crystallographica Section D Biological Crystallography. 52 (3): 435-46.