CASP3 / Caspase-3 / CASP-3 Protein (His Tag)

13.06.2013

Sino Biological Inc. liefert CASP3 / Caspase-3 / CASP-3 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CASP3 / Caspase-3 / CASP-3 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/Caspase-3-CASP-3-Protein-Antibody-a-6234.html

Produkt-Information von CASP3 / Caspase-3 / CASP-3 Protein

Synonym	Caspase3, CASP3
Protein Construction	A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CASP3 / Caspase-3 / CASP-3 Protein

Purity	(48.4+48.1) % as determined by SDS-PAGE	SDS-PAGE: CASP3 protein
Bio-activity	Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is > 5000 pmoles/min/μg.
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CASP3 / Caspase-3 / CASP-3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CASP3 / Caspase-3 / CASP-3 Protein

Caspase-3, also known as CASP-3, is a member of the cysteine-aspartic acid protease (caspase) family. It is highly expressed in lung, spleen, heart, liver and kidney. Caspase-3 also can be detected in many cell lines. Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase-3 also cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. It cleaves and activates caspase-6, -7 and -9. Caspase-3 can triggers cell adhesion in sympathetic neurons through RET cleavage.

References

Perry DK. et al., 1997, J Biol Chem. 272 (30): 18530-3.
Harrington HA. et al., 2008, Theor Biol Med Model. 5 (1): 26.
Alnemri ES. et al., 1996, Cell. 87 (2): 171.