Cynomolgus IL2RA Protein (His Tag)

13.06.2013

Sino Biological Inc. liefert Cynomolgus IL2RA Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus IL2RA Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CD25-IL2RA-Protein-Antibody-a-302.html

Produkt-Information von IL2RA Protein

Synonym	IL2RA
Protein Construction	A DNA sequence encoding the cynomolgus IL2RA (H6WS54) (Met1-Arg213) was expressed with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von IL2RA Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: IL2RA protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 22
Molecular Mass	The recombinant cynomolgus IL2RA comprises 203 amino acids and has a calculated molecular mass of 23.1 KDa. The apparent molecular mass of it is approximately 43 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von IL2RA Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IL2RA Protein

Interleukin 2 receptor alpha (IL2Rα), also known as CD25 molecule, is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2Rα / CD25 is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2Rα is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. Accordingly, IL2 receptor complex mediates the IL2 effects via regulating T cell proliferation, activation and other lymphoid functions, and IL2Rα has been shown to deliver IL2 to the signaling complex and act as a regulator of signal transduction. In addition, IL2 induces IL2R transcription via an IL2-responsive enhancer, and IL2R also inhibits IL2 gene expression by activating the expression of CTLA-4 or FOXP3 via the negative feedback loops.

References

Purvis SF. et al., 1992, Cell Immunol. 144: 32-42.
Rusterholz C. et al., 1999, Mol Cell Biol. 19: 2681-9.
Kim HP. et al., 2005, J Biol Chem. 280: 34042-7.
Stauber DJ. et al., 2006, Proc Natl Acad Sci. 103: 2788-93.
Fragale A. et al., 2008, J Immunol. 181: 1673-82.