CARHSP1 Protein (His Tag)

06.05.2013

Sino Biological Inc. liefert CARHSP1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CARHSP1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CARHSP1-Protein-Antibody-a-5930.html

Produkt-Information von CARHSP1 Protein

Synonym	CARHSP1
Protein Construction	A DNA sequence encoding the human CARHSP1(Q9Y2V2 ) (Met1-Ser147) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CARHSP1 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE CARHSP1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CARHSP1 consists of 162 amino acids and predicts a molecular mass of 17.7 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tirs, 10% Glycerol, 200mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CARHSP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CARHSP1 Protein

CARHSP1 is a biomarker for diabetic complications. Adenovirus-mediated CARHSP1 overexpression and siRNA-mediated knockdown experiments were performed to characterize the role of CARHSP1 in the regulation of gluconeogenic gene expression. CARHSP1 is regulated by nutrient status in the liver and functions at the transcriptional level to negatively regulate gluconeogenic genes, including the glucose-6-phosphatase catalytic subunit (G6Pc) and phosphoenolpyruvate carboxykinase 1 (PEPCK1). In addition, it is found that CARHSP1 can physically interact with peroxisome proliferator-activated receptor-α (PPARα) and inhibit its transcriptional activity. Both pharmacological and genetic ablations of PPARα attenuate the inhibitory effect of CARHSP1 on gluconeogenic gene expression in hepatocytes.

References

Wistow G. et al., 2002, Mol Vis. 8: 205-20.
Wishart MJ. et al., 2002, Proc Natl Acad Sci. 99 (4): 2112-7.
Groblewski GE. et al., 1998, J Biol Chem. 273 (35): 22738-44.
Fan Y. et al., 2011, J Biol Chem. 286 (47): 40584-94.