Carbonic Anhydrase IX / CA9 Antikörper

08.05.2012

Produkt-Information von Carbonic Anhydrase IX / CA9 Antibody

Immunogen	Recombinant Human Carbonic Anhydrase IX / CA9 protein (Catalog#10107-H08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	005
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; Catalog#10107-H08H; NP_001207.2; Met 1-Asp 414).

Usage Guide von Carbonic Anhydrase IX / CA9 Antikörper

Specificity

Human Carbonic Anhydrase IX / CA9

Immunochemistry

IHC-P: 1-5 μg/mL

CA9-stomach immunohistochemistry CA9-gastric-cancer immunohistochemistry

Immunochemical staining of human CA9 in human stomach with rabbit monoclonal antibody (2 μg/mL, formalin-fixed paraffin embedded sections).

Immunochemical staining of human CA9 in human gastric cancer with rabbit monoclonal antibody (2 μg/mL, formalin-fixed paraffin embedded sections).

CA9-bladder-carcinoma immunohistochemistry CA9-renal-carcinoma immunohistochemistry

Immunochemical staining of human CA9 in human bladder carcinoma with rabbit monoclonal antibody (5μg/mL, formalin-fixed paraffin embedded sections).

Immunochemical staining of human CA9 in human renal carcinoma with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections).

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CA9 in WB.

Direct ELISA

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA9. The detection limit for Human CA9 is approximately 0.00245 ng/well.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von Carbonic Anhydrase IX / CA9 Antikörper

Related Areas:

Cancer>>Cancer Biomarkers>>Carbonic Anhydrase IX

Enzyme>>Carbonic Anhydrase>>Carbonic Anhydrase IX

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
Carbonic Anhydrase IX	Human	Carbonic Anhydrase IX/Fc Protein, Recombinant	10107-H02H
Carbonic Anhydrase IX	Human	Carbonic Anhydrase IX Protein, Recombinant	10107-H08H
Carbonic Anhydrase IX	Mouse	Carbonic Anhydrase IX Protein, Recombinant	50660-M08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human Carbonic Anhydrase IX	WB, ELISA	Rabbit Monoclonal Antibody	10107-R003
Human Carbonic Anhydrase IX	WB, ELISA, IHC-P	Carbonic Anhydrase IX / CA9 Antibody	10107-R005
Human Carbonic Anhydrase IX	ELISA	Rabbit Polyclonal Antibody	10107-RP01
Human Carbonic Anhydrase IX	WB, ELISA, IHC-P	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10107-RP02
Mouse Carbonic Anhydrase IX	WB, ELISA,	Carbonic Anhydrase IX / CAR9 Antibody	50660-RP01
Mouse Carbonic Anhydrase IX	WB, ELISA	Carbonic Anhydrase IX / CAR9 Antibody (Antigen Affinity Purified)	50660-RP02

Hintergrund von Carbonic Anhydrase IX / CA9 Antikörper

Carbonic anhydrases IX (CAIX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CAIX is a transmembrane protein structurally consisting of a signal peptide, a proteoglycan-related region, a CA domain with a highly conserved active site, a transmembrane anchor and an intracytoplasmic tail, and is the only tumor-associated CA isoenzyme known so far. Compared with normal tissues, CAIX is overexpressed in a wide spectrum of tumor types and associated with increased metastasis and poor prognosis in aggressive carcinomas. CAIX expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. CA9 is thus regarded as a new therapeutic target for CA9-derived carcinomas.

References

Pastorek, J. et al., 1994, Oncogene. 9: 2877-2888.
Opavsky, R. et al., 1996, Genomics. 33: 480-487.
Swietach, P. et al., 2008, J. Biol. Chem. 283: 20473-20483.
Robertson, N. et al., 2004, Cancer. Res. 64: 6160-6165.
Bui, M.H. et al., 2003, Clin. Cancer. Res. 9: 802-811.
Choi, S.W. et al., 2008, Hum. Pathol. 39: 1317-1322.

Sino Biological Inc. liefert Carbonic Anhydrase IX / CA9 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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