Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper

21.05.2012

Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper

Immunogen	Recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin protein (Catalog#10204-H08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	7G5D4
Ig Type	Mouse IgG1
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper

Specificity

Human Cadherin-1 / CDH1 / CD324 / E-cadherin

Immunochemistry

ICC/IF: 15-30 μg/mL

E-cadherin-MM04-15ug-MCF-7-IF Immunofluorescence

Immunofluorescence staining of human E-cadherin in MCF-7 cells with mouse monoclonal antibody (15 μg/mL). The image showing membrane staining of MCF-7 cells.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human E-cad in WB.

Direct ELISA

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human E-cad.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper

Related Areas:

Cardiovascular>>Angiogenesis>>Adhesion Molecules in Angiogenesis>>E-Cadherin/CDH1/CD324

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Hemangioblast Marker>>E-Cadherin/CDH1/CD324

Stem Cell>>Embryonic Stem Cell (ESC)>>Embryonic Stem Cell Marker>>E-Cadherin/CDH1/CD324

Cancer>>Cancer Stem Cell (CSC)>>E-Cadherin/CDH1/CD324

Cancer>>Angiogenesis>>Adhesion Molecules in Angiogenesis>>E-Cadherin/CDH1/CD324

Cancer>>Cancer Biomarkers>>E-Cadherin/CDH1/CD324

Immunology>>Adhesion Molecule>> Cadherin>>E-Cadherin/CDH1/CD324

Immunology>>Innate Immunity>>Natural Killer Cell (NK Cell)>>E-Cadherin/CDH1/CD324

Immunology>>Cluster of Differentiation>>Other>>E-Cadherin/CDH1/CD324

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
E-Cadherin/CDH1/CD324	Human	E-Cadherin/CDH1 Protein, Recombinant	10204-H08H
E-Cadherin/CDH1	Mouse	E-Cadherin/CDH1 Protein, Recombinant	50671-M08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human E-Cadherin/CDH1/CD324	WB, ELISA, ICC/IF, IF	Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody	10204-MM04
Human E-Cadherin/CDH1/CD324	WB, ELISA	Mouse Monoclonal Antibody	10204-MM07
Human E-Cadherin/CDH1/CD324	FCM	Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody ( FITC )	10204-MM08-F
Human E-Cadherin/CDH1/CD324	ELISA	Mouse Monoclonal Antibody	10204-MM12
Human E-Cadherin/CDH1/CD324	WB, ELISA	Rabbit Polyclonal Antibody	10204-RP01
Human E-Cadherin/CDH1/CD324	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10204-RP02
Mouse E-Cadherin/CDH1/CD324	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50671-RP02

Hintergrund von Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).

References

Berx G. et al., 1995, Genomics 26: 281-289.
Yoshiura K. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 7416-7419.
Becker K.-F. et al., 1994, Cancer Res. 54: 3845-3852.
Marambaud P. et al., 2002, EMBO J. 21: 1948-1956.
Steinhusen U. et al., 2001, J. Biol. Chem. 276: 4972-4980.

Sino Biological Inc. liefert Cadherin-1 / CDH1 / CD324 / E-cadherin Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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