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Carbonic Anhydrase IV / CA4 Antikörper (Antigen Affinity Purified)

Produkt-Information von Carbonic Anhydrase IV / CA4 Antikörper

Immunogen

Recombinant Human Carbonic Anhydrase IV / CA4 protein (Catalog#10472-H08H)

Antibody Type Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type

Rabbit IgG

Formulation 0.2 μm filtered solution in PBS with 5% trehalose
Preparation

Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase IV / CA4 (rh Carbonic Anhydrase IV / CA4; Catalog#10472-H08H; NP_667341.1; Met 1-Lys 283). Carbonic Anhydrase IV / CA4 specific IgG was purified by Human Carbonic Anhydrase IV / CA4 affinity chromatography.

Usage Guide von Carbonic Anhydrase IV / CA4 Antikörper

Specificity

Human Carbonic Anhydrase IV / CA4

Immunochemistry

IHC-P 0.5-2 μg/mL

 

CA4-lung immunohistochemistry    CA4-kidney immunohistochemistry

Immunochemical staining of human CA4 in human lung with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to pulmonary capillaries. Immunochemical staining of human CA4 in human kidney with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to renal tubules.
Western blot This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA4 in WB. Using a DAB detection system, the detection limit for Human CA4 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CA4.
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von Carbonic Anhydrase IV / CA4 Antikörper

Related Areas:

Enzyme>>Carbonic Anhydrase>>Carbonic Anhydrase IV

Proteins:
Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
Carbonic Anhydrase IV Human Carbonic Anhydrase IV Protein, Recombinant 10472-H08H
Carbonic Anhydrase IV Mouse Carbonic Anhydrase IV Protein, Recombinant 50350-M08H
Antibodies:
Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human Carbonic Anhydrase IV WB, ELISA Carbonic Anhydrase IV / CA4 Antibody (Antigen Affinity Purified) 10472-RP02

Hintergrund von Carbonic Anhydrase IV / CA4 Antikörper

Carbonic anhydrase 4, also known as Carbonic anhydrase IV, CAIV, and CA4, is a member of the alpha-carbonic anhydrase family. CA4 is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. It is expressed on the luminal surfaces of pulmonary capillaries and of proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport. Similar to human CA4, the C-terminus of murine CA4 is surrounded by a substantial electropositive surface potential that may stabilize the interaction with the phospholipid membrane. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. The genomic data suggests that there are thirteen enzymatically active members of the mammalian CA isozyme family. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization.

References

  1. Okuyama, T. et al., 1993, Genomics16 (3): 678-84.
  2. Stams, T.et al., 1998, Protein Sci. 7: 556-563.
  3. Sterling, D. et al., 2002, J. Biol. Chem. 277 (28): 25239-46.
  4. Pan, PW. et al., 2007, BMC Mol Biol. 8: 22.

Sino Biological Inc. liefert Carbonic Anhydrase IV / CA4 Antikörper (Antigen Affinity Purified) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Carbonic Anhydrase IV / CA4 Antikörper (Antigen Affinity Purified) lesen Sie bitte: http://www.sinobiological.com/Carbonic-Anhydrase-IV-CA4-Antibody-g-8863.html

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