BPI Protein (His Tag)

19.09.2012

Produkt-Information von BPI Protein

Synonym	BPI
Protein Construction	A DNA sequence encoding the human BPI (P17213) (Met1-Lys487) with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von BPI Protein

Purity	> 98 % as determined by SDS-PAGE	SDS-PAGE: BPI protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 32
Molecular Mass	The recombinant human BPI comprises 467 amino acids and has a predicted molecular mass of 52.1 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von BPI Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von BPI Protein

Bactericidal/permeability-increasing protein belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It is a lipopolysaccharide binding protein associated with human neutrophil granules and has bactericidal activity on gram-negative organisms. The cytotoxic action of bactericidal/permeability-increasing protein is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It also has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Bactericidal/permeability-increasing protein contains two domains that adopt the same structural fold, even though they have little sequence similarity. It is a potent antimicrobial protein of 456 residues that binds to and neutralises lipopolysaccharides from the outer membrane of Gram-negative bacteria.

References

G Schlag. et al., 1999, Annals of Surgery. 229 (2): 262-71.
Michael Levin. et al., 2000, Lancet. 356 (9234): 961-7.
Geraldine Canny. et al., 2002, PNAS. 99 (6): 3902-7.

Sino Biological Inc. liefert BPI Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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