MBD2 / DPEP2 Protein (His Tag)

14.03.2013

Sino Biological Inc. liefert MBD2 / DPEP2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über MBD2 / DPEP2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/MBD2-DPEP2-Protein-g-10606.html

Produkt-Information von MBD2 / DPEP2 Protein

Synonym	DPEP2
Protein Construction	A DNA sequence encoding the human DPEP2 (AAH24021.1) (Met1-Ser376) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von MBD2 / DPEP2 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: MBD2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 33
Molecular Mass	The recombinant human DPEP2 consists of 355 amino acids and predicts a molecular mass of 39.4 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von MBD2 / DPEP2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MBD2 / DPEP2 Protein

MBD2, also known as DPEP2, is a nuclear protein. It belongs to a family related by the presence in each of a methyl-CpG-binding domain (MBD). Besides MBD2, the family members also include MECP2, MBD1, MBD3, and MBD4. Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1, and MBD2 can also repress transcription from methylated gene promoters. It is reported that MBD2 functions as a demethylase. MBD2 may function as a mediator of the biological consequences of the methylation signal.

References

Hendrich B. et al., 1999, Mamm Genome. 10 (9): 906-12.
Brackertz. et al., 2002, J Biol Chem. 277 (43): 40958-66.
Zhang Y. et al., 1999, Nat Genet. 23 (1): 58-61.