BMP-2 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 1.0 mg/mL of mouse anti-BMP2 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-BMP2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 100 ng of recombinant BMP2. Reconstitute standard powder with detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.

The minimum detectable dose of Human BMP-2 was determined to be approximately 78 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human BMP-2 ELISA Pair Set is for the quantitative determination of Human BMP-2.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for BMP-2 coated on a 96-well plate. Standards and samples are added to the wells, and any BMP-2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-BMP-2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of BMP-2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Bone morphogenetic protein 2 (BMP2) is a member of the BMP subgroup belonging to the transforming growth factor (TGF)-beta superfamily. The mature BMP2 acts as disulfide-linked homodimers and heterodimers with BMP-7 after proteolytic removal of propeptide. BMP2 is capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. It binds heterodimeric receptor complexes composed of a type I receptor and a type I I receptor, and transduces the related signals via the down-stream molecules, such as the PI3 kinase, Smads, STATs, etc. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function. In addition, recent studies have revealed that osteoblast-derived BMP2 enhances the migration of prostate cancer cells. rhBMP2 can be utilized in various therapeutic interventions such as bony defects, delayed union, non-union fractures and osteoporosis.