TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.2 mg/mL of rabbit anti-TIMD3 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.25 mg/mL mouse anti-TIMD3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 70 ng of recombinant TIMD3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.

The minimum detectable dose of Human TIM-3 / HAVCR2 / TIMD3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set is for the quantitative determination of Human TIM-3 / HAVCR2 / TIMD3.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for TIM-3 / HAVCR2 / TIMD3 coated on a 96-well plate. Standards and samples are added to the wells, and any TIM-3 / HAVCR2 / TIMD3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-TIM-3 / HAVCR2 / TIMD3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of TIM-3 / HAVCR2 / TIMD3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Hepatitis A virus cellular receptor 2 ( HAVCR2 ), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. TIM-3 thus serves as the surface molecule that distinguishes between the two effector populations derived from naive CD4(+) T-helper cells. TIM-3 is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. Th1 cells produce cytokines ( IFN-gamma, interleukin-2, TNF-alpha and lymphotoxin ) that are commonly associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity reactions, and induction of organ-specific autoimmune diseases. It has been identified that galectin-9 is the Tim-3 ligand, and the TIM-3-galectin-9 pathway may serve to inhibit Th1 cells during a normal immune response and may be crucial for the induction of peripheral tolerance. Dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.