CD38 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 1 mg/mL of rabbit anti-CD38 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.

Nachweisantikörper 1 mg/mL rabbit anti-CD38 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.2 μg/mL in detection antibody dilution buffer before use.

Standard Each vial contains 140 ng of recombinant CD38. Reconstitute standard powder with detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.

The minimum detectable dose of Human CD38 was determined to be approximately 39.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human CD38 ELISA Pair Set is for the quantitative determination of Human CD38.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD38 coated on a 96-well plate. Standards and samples are added to the wells, and any CD38 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD38 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD38 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

CD38 (cluster of differentiation 38), also known as ADP-ribosyl cyclase, is a glycoprotein expressed on the surface of immune cells including B and T lymphocytes, osteoclasts, as well as cardiac, pancreatic, liver and kidney cells. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic β cells. CD38 functions in cell adhesion, signal transduction and calcium signaling. Defects of CD38 is associated with impaired immune responses, metabolic disturbances, and behavioral modifications, and thus has been connected to HIV infection, leukemias, myelomas, solid tumors, type I I diabetes mellitus and bone metabolism, as well as some genetically determined conditions.