Mouse Prolyl endopeptidase / PREP Protein (His Tag)

11.01.2013

Sino Biological Inc. liefert Mouse Prolyl endopeptidase / PREP Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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Produkt-Information von Prolyl endopeptidase / PREP Protein

Synonym	PREP
Protein Construction	A DNA sequence encoding the mature form of mouse PREP (Q9QUR6) (Leu2-Gln710) was expressed, with a polyhistidine tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von Prolyl endopeptidase / PREP Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PREP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant mouse PREP consists of 727 amino acids and predicts a molecular mass of 82.9 KDa. It migrates as an approximately 79-83 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4,10% glycerol, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Prolyl endopeptidase / PREP Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Prolyl endopeptidase / PREP Protein

Prolyl endopeptidase, also known as PREP, is a serine peptidase. It also is a large cytosolic enzyme which was first described in the cytosol of rabbit brain as an oligopeptidase. Prolyl endopeptidase’s activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. It degrades the nonapeptide bradykinin at the Pro-Phe bond. It is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimulating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. Prolyl endopeptidase cleaves peptide bonds at the C-terminal side of proline residues.

References

Oliveira EB. et al., 1976, Biochemistry. 15 (9): 1967-74.
Stepniak D. et al., 2006, Am J Physiol Gastrointest Liver Physiol. 291 (4): G621-9.
Jarho EM. et al., 2007, Bioorg Med Chem. 15 (5): 2024-31.