AKR1C2 Protein (His Tag)

06.05.2013

Sino Biological Inc. liefert AKR1C2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über AKR1C2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/AKR1C2-DDH2-Protein-Antibody-a-5910.html

Produkt-Information von AKR1C2 Protein

Synonym	AKR1C2, DDH2
Protein Construction	A DNA sequence encoding the mature form of human AKR1C2 (P52895-1) (Met1-Tyr323) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von AKR1C2 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: AKR1C2 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human AKR1C2 consists of 338 amino acids and predicts a molecular mass of 38.6 KDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von AKR1C2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von AKR1C2 Protein

AKR1C2 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. AKR1C2 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for AKR1C2 gene.

References

Jin Y. et al., 2011, Biochem J. 437 (1): 53-61.
Veilleux A. et al., 2012, Am J Physiol Endocrinol Metab. 302 (8): E941-9.
Kuang P. et al., 2012, Lung Cancer. 77 (2): 427-32.