ADAMTSL1 / PUNCTIN Protein (His Tag)

09.10.2012

Produkt-Information von ADAMTSL1 / PUNCTIN Protein

Synonym	ADAMTSL1, ADAMTSR1, C9orf94
Protein Construction	A DNA sequence encoding the human ADAMTSL1 ( Met 1- His439) (Q8N6G6 -2) was expressed, with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ADAMTSL1 / PUNCTIN Protein

Purity	> 97 % as determined by SDS-PAGE	SDS-PAGE: ADAMTSL1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ile 24
Molecular Mass	The secreted recombinant human ADAMTSL1 consists of 421 amino acids and predicts a molecular mass of 47 KDa. The apparent molecular mass of the protein is approximately 48 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ADAMTSL1 / PUNCTIN Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ADAMTSL1 / PUNCTIN Protein Related Products & Topics

Related Areas:

Proteins:

Antibodies:

Beschreibung von ADAMTSL1 / PUNCTIN Protein

ADAMTSL1 is a secreted molecule. It is similar to the members of the ADAMTS protein family which is composed by matrix metalloproteinases. Both ADAMTS proteins and ADAM protein family contain a disintegrin and a metalloprotease domain. While ADAMTSL1 contains the domains found in members of the ADAMTS protein family with the exception of the pro-metalloprotease and the disintegrin-like domain typical of this family. ADAMTSL1 gene is expressed in adult skeletal muscle. ADAMTSL1 may play an important role in the extracellular matrix as it is deposited in the cell substratum in a punctate fashion and is excluded from focal contacts. a long alternatively spliced isoform of this protein have been identified with additional thrombospondin-like repeats, Ig-like modules, and a C-terminal PLAC domain. It has been reported that N-glycosylation and O-fucosylation modify the secretion of ADAMTSL1, and its absence can downregulation secretion of this protein.

References

Hirohata S. et al., 2002, J Biol Chem. 277 (14): 12182-9.
Wang LW. et al., 2007, J. Biol. Chem. 282 (23): 17024-31.
Hall NG. et al., 2004, Matrix Biol. 22 (6): 501-10.

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