ABHD10 (aa 53-306) Protein (His Tag)

11.04.2013

Sino Biological Inc. liefert ABHD10 (aa 53-306) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ABHD10 (aa 53-306) Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ABHD10-Protein-Antibody-a-5773.html

Produkt-Information von ABHD10 Protein

Synonym	ABHD10
Protein Construction	A DNA sequence encoding the human ABHD10 (Q9NUJ1)(Thr53-Asn306) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ABHD10 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ABHD10 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ABHD10 consists of 271 amino acids and has a calculated molecular mass of 30.3 kDa. The recombinant protein migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ABHD10 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ABHD10 Protein

Mycophenolic acid (MPA), the active metabolite of the immunosuppressant mycophenolate mofetil (MMF), is primarily metabolized by glucuronidation to a phenolic glucuronide (MPAG) and an acyl glucuronide (AcMPAG). It is known that AcMPAG, which may be an immunotoxic metabolite, is deglucuronidated in human liver. AcMPAG deglucuronidation activity was detected in both human liver cytosol (HLC) and microsomes (HLM). By purification from HLC with column chromatographic purification steps, the enzyme responsible for AcMPAG deglucuronidationis identified as α/β hydrolase domain containing 10 (ABHD10). Recombinant ABHD10 expressed in Sf9 cells efficiently deglucuronidated AcMPAG with a K(m) value of 100.7 ± 10.2 μM, which was similar to those in HLM, HLC, and human liver homogenates (HLH). Immunoblot analysis revealed ABHD10 protein expression in both HLC and HLM. The AcMPAG deglucuronidation by recombinant ABHD10, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB. The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF. Thus, human ABHD10 would affect the formation of AcMPAG, the immunotoxic metabolite.

References

Nardini M. et al., 1999, Curr Opin Struct Biol. 9 (6): 732-7.
Carr PD. et al., 2009, Protein Pept Lett. 16 (10): 1137-48.
Cheah E. et al., 1992, Protein Eng. 5 (3): 197-211.
Iwamura A. et al., 2012, J Biol Chem. 287 (12): 9240-9.

ABHD10 related areas, pathways, and other information