VRK1 Protein (GST Tag)

04.05.2012

Produkt-Information von VRK1 Protein

Synonym	PCH1
Protein Construction	A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von VRK1 Protein

Purity	> 88 % as determined by SDS-PAGE	SDS-PAGE: VRK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human VRK1/GST chimera consists of 633 amino acids and has a calculated molecular mass of 73 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10%gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von VRK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von VRK1 Protein

VRK1 belongs to the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. VRK1 localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. VRK1 gene, therefore, may regulate cell proliferation. VRK1 also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. VRK1 is also involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. It acts by mediating phosphorylation of downstream target protein. Defects in VRK1 can cause of pontocerebellar hypoplasia type 1 which is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

References

Sugimoto J. et al., 1999, J Hum Genet. 44 (2): 133-4.
Lopez-Borges S. et al., 2000, Oncogene. 19( 32): 3656-64.
Sevilla A. et al., 2004, Oncogene. 23 (55): 8950-8.

Sino Biological Inc. liefert VRK1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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