UBCH8 / UBE2L6 Protein (His Tag)

06.06.2012

Produkt-Information von UBCH8 / UBE2L6 Protein

Synonym	RIG-B, UBCH8
Protein Construction	A DNA sequence encoding the human UBE2L6 isoform 1(O14933-1) (Met 1-Ser 153) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von UBCH8 / UBE2L6 Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: UBCH8 / UBE2L6 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human UBE2L6 consisting of 168 amino acids and has a calculated molecular mass of 19.6KDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation	Lyophilized from Lyophilizedfroma0.2μmfilteredsolutionof20mMTris, 0.1%Brij35; pH8.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von UBCH8 / UBE2L6 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von UBCH8 / UBE2L6 Protein

UBCH8, also known as UBE2L6, is a member of the ubiquitin-conjugating enzyme family. UBCH8 functions in ubiquitination. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBCH8 is a member of the E2 ubiquitin-conjugating enzyme family. It is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. UBCH8 catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. It functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.

References

Moynihan TP. et al., 1999, J Biol Chem. 274 (43): 30963-8.
Ardley HC. et al., 2000, Cytogenet Cell Genet. 89 (1-2): 137-40.
Zhao C. et al., 2004, Proc Natl Acad. 101 (20): 7578-82.
Tripathi MK. et al., 2005, Biochem Biophys Res Commun. 328 (1): 43-8.

Sino Biological Inc. liefert UBCH8 / UBE2L6 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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