IFITM3 Protein

06.06.2012

IFITM3 Protein Product Information

Synonym	IFITM3
Protein Construction	A DNA sequence encoding the human IFITM3(NP_066362.2) (Met1-His57) was expressed, fused with the Fc region of mouse IgG1 at the N-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von IFITM3 Protein

Purity	> 99 % as determined by SDS-PAGE	SDS-PAGE: IFITM3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp
Molecular Mass	The recombinant human IFITM3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 293 amino acids and has a predicted molecular mass of 32.9 kDa. The apparent molecular mass of the monomer is approximately 33-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS,pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IFITM3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IFITM3 Protein

Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM3 is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. Notably, viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.

References

Tanaka SS. et al., 2005, Dev Cell. 9 (6): 745-56.
Li D. et al., 2011, Clin Cancer Res. 17 (11): 3558-68.
Lu J. et al., 2011, J Virol. 85 (5): 2126-37.

Sino Biological Inc. liefert IFITM3 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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