TLR4 / CD284 Protein (His Tag)

17.09.2012

Produkt-Information von TLR4 / CD284 Protein

Synonym	TLR4
Protein Construction	A DNA sequence encoding the human TLR4 ( Met 1- Lys631) (O00206-1) was expressed, with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von TLR4 / CD284 Protein

Purity	> 87 % as determined by SDS-PAGE	SDS-PAGE: TLR4 / CD284 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 24
Molecular Mass	The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von TLR4 / CD284 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TLR4 / CD284 Protein

TLR4, also known as TLR-4, belongs to the Toll-like receptor (TLR) family. TLR 4 has also been designated as CD284 (cluster of differentiation 284). TLR family plays a fundamental role in pathogen recognition and activation of innate immunity. TLR4 is highly expressed in placenta, spleen and peripheral blood leukocytes. It also can be detected in monocytes, macrophages, dendritic cells and several types of T-cells. It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 also is involved in LPS-independent inflammatory responses triggered by Ni(2+). Genetic variation in TLR4 is associated with age-related macular degeneration type 10. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world.

References

Re Fabio. et al., 2002, J Biol Chem. 277 (26): 23427-32.
Shimazu R. et al., 1999, J Exp Med. 189 (11): 1777-82.
Espevik T. et al., 2010, Immunity. 33 (4): 583-96.
Blanco A M. et al., 2005, Journal of immunology. 175 (10): 6893-9.

Sino Biological Inc. liefert TLR4 / CD284 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über TLR4 / CD284 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/TLR4-CD284-Protein-g-9285.html