TLR2 / CD282 (aa 1-587) Protein (His Tag)

04.05.2012

Produkt-Information von TLR2 / CD282 Protein

Synonym	CD282, TIL4
Protein Construction	A DNA sequence encoding the human TLR2 (O60603) extracellular domain (Met 1-Arg 587) was expressed, with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von TLR2 / CD282 Protein

Purity	> 88 % as determined by SDS-PAGE	SDS-PAGE: TLR2 / CD282 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 19
Molecular Mass	The secreted recombinant human TLR2 consists of 580 amino acids and predicts a molecular mass of 65.8kDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10%gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von TLR2 / CD282 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TLR2 / CD282 Protein

TLR2, also known as CD282, belongs to the Toll-like receptor (TLR) family. Toll-like receptors (TLRs) are single transmembrane cell-surface receptors, which have a key role in the innate immune system. TLRs generally exist as homodimers and are found on immune cells such as macrophages, B lymphocytes and mast cells. Toll-like receptors are activated by molecules associated with biological threat and are highly specific towards evolutionary conserved entities on microbes, such as bacterial cell-surface lipopolysaccharides, flagella and unmethylated CpG islands. TLR2 is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. It is also detected in lung and in fetal liver. Levels are low in other tissues. TLR2 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It also cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. TLR2 may also promote apoptosis in response to lipoproteins. CD282 recognizes mycoplasmal macrophage-activating lipopeptide-2kD, soluble tuberculosis factor, phenol-soluble modulin and B.burgdorferi outer surface protein A lipoprotein cooperatively with TLR6.

References

Do KN. et al., 2012, PLoS ONE. 7 (3): e32309.
Dziarski R. et al., 2001, J Endotoxin Res. 6( 5): 401-5.
Lorenz E. 2007, Curr Pharm Des. 12 (32): 4185-93.

Sino Biological Inc. liefert TLR2 / CD282 (aa 1-587) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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