CHK1 Protein (GST Tag)

04.05.2012

Produkt-Information von CHK1 Protein

Synonym	CHEK1
Protein Construction	A DNA sequence encoding the human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CHK1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CHK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CHK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CHK1 Protein

CHK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. CHK1 is expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 may also negatively regulate cell cycle progression during unperturbed cell cycles. It recognizes the substrate consensus sequence [R-X-X-S/T] and binds to and phosphorylates CDC25A, CDC25B and CDC25C. CHK1 binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. It also binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. CHK1 may also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

References

Chen P. et al., 2000, Cell. 100 (6): 681-92.
Sanchez Y. et al., 1997, Science. 277 (5331): 1497-501.
Flaggs G. et al., 1998, Curr Biol. 7 (12): 977-86.
Chini CC. et al., 2005, DNA Repair. 3 (8-9): 1033-7.

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