Thioredoxin / TXN Antikörper (Antigen Affinität reinigt)

13.03.2012

Produkt-Information von Thioredoxin / TXN Antikörper

Immunogen	Recombinant Human Thioredoxin / TXN protein (Catalog#10384-HNAE)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; Catalog#10384-HNAE; P10599; Met 1-Val 105). Thioredoxin / TXN specific IgG was purified by Human Thioredoxin / TXN affinity chromatography.

Thioredoxin / TXN Antikörper Usage Guide

Specificity	Human Thioredoxin / TXN
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TXN in WB. Using a DAB detection system, the detection limit for Human TXN is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TXN. The detection limit for Human TXN is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Thioredoxin / TXN Antikörper

Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to thethioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

References

Holmgren A, et al.,1989, J Biol Chem264 (24): 13963-6.
Nordberg J, et al., 2001,Free Radic Biol Med31 (11): 1287-312.
Mustacich D, et al., 2000, Biochem J346 (Pt 1): 1-8.
Mitchell D.A.,et al., 2005, Nat. Chem. Biol. 1:154-158.

Sino Biological Inc. liefert Thioredoxin / TXN Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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