TGFBR1 / ALK-5 / SKR4 (aa 200-503) Protein (GST Tag)

07.03.2013

Sino Biological Inc. liefert TGFBR1 / ALK-5 / SKR4 (aa 200-503) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über TGFBR1 / ALK-5 / SKR4 (aa 200-503) Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/TGFBR1-ALK-5-SKR4-Protein-g-10549.html

Produkt-Information von TGFBR1 / ALK-5 / SKR4 Protein

Synonym	ALK5, TGFBR1, SKR4
Protein Construction	A DNA sequence encoding the human ALK5 (P36897-1) (Thr200-Met503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von TGFBR1 / ALK-5 / SKR4 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: TGFBR1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human ALK5/GST chimera consists of 541 amino acids and has a calculated molecular mass of 62.6 kDa. The recombinant protein migrates approximately 57 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5,10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von TGFBR1 / ALK-5 / SKR4 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TGFBR1 / ALK-5 / SKR4 Protein

TGF-beta receptor type-1, also known as Transforming growth factor-beta receptor type I , Serine/threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. On ligand binding, TGFBR1 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).

References

De Gouville AC. et al., 2006, Drug News Perspect. 19 (2): 85-90.
Seki T. et al., 2006, Lab Invest. 86 (2): 116-29.
Frazier K. et al., 2007, Toxicol Pathol. 35 (2): 284-95.
Sridurongrit S. et al., 2008, Dev Biol. 322 (1): 208-18.
Zhao H. et al., 2008, Dev Biol. 320 (1): 19-29.

TGF-beta