Mouse SMAD2 Protein (GST Tag)

07.03.2013

Sino Biological Inc. liefert Mouse SMAD2 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse SMAD2 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/SMAD2-Protein-g-10552.html

Produkt-Information von SMAD2 Protein

Synonym	SMAD2, Madh2, Madr2
Protein Construction	A DNA sequence encoding the mouse SMAD2 (Q62432-Isoform Long) (Ser2-Ser467) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von SMAD2 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SMAD2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse SMAD2/GST chimera consists of 703 amino acids and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8.0, 10% glycerol, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SMAD2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SMAD2 Protein

SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.

References

Feng. et al., 2002, Mol Cell. 9 (1): 133-43.
Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40.
Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.

Cytokine

TGF-beta